<i>N</i>–nitrosodiethylamine cytochrome P450 induction and cytotoxicity evaluation in primary cultures of rat hepatocytes

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N–nitrosodiethylamine cytochrome P450 induction and cytotoxicity evaluation in primary cultures of rat hepatocytes

The primary routes of potential human exposure to N-nitrosodiethylamine (NDEA) are ingestion, inhalation, and dermal contact. Air, diet and smoking contribute to potential human exposure at levels of a few μg of NDEA/day. Potential exposure depends on the ability of the nitrosamines to migrate from the product into the body. The first step in the metabolic degradation of NDEA by cytochrome oxid...

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Cytotoxicity of mitomycin C and adriamycin in freshly isolated rat hepatocytes: the role of cytochrome P450.

The role of cytochrome P450 (P450) in the cytotoxicity of mitomycin C (MMC) and Adriamycin (ADR) was investigated in freshly isolated hepatocytes from phenobarbital-induced rats. The loss of cell viability [measured as lactate dehydrogenase (LDH) leakage] upon MMC exposure was accompanied by a rapid and extensive intracellular glutathione (GSH) depletion and followed by minor lipid peroxidation...

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ژورنال

عنوان ژورنال: American Journal of Molecular Biology

سال: 2011

ISSN: 2161-6620,2161-6663

DOI: 10.4236/ajmb.2011.12009